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Test Description
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Semen Analysis

A semen analysis measures the amount of semen and determines the number and quality of sperm.

A semen analysis is usually one of the first tests done to help determine whether a man has a problem that is making it difficult for him to father a child. A problem with the semen or sperm affects about one-third of the couples who are unable to have children (infertile).

Tests that may be done during a semen analysis include:

  • Volume. This is a measure of how much semen is present in one ejaculation.
  • Liquefaction time. Semen is a thick gel at the time of ejaculation, but normally becomes liquid about 20 minutes after ejaculation. Liquefaction time is a measure of the time it takes for the semen to liquefy.
  • Sperm count. This is a count of the number of sperm present per milliliter of semen in one ejaculation.
  • Sperm morphology. This is a measure of the percentage of sperm that have a normal shape.
  • Sperm motility. This is a measure of the percentage of sperm that can move forward normally. The number of sperm that show normal forward movement in a certain amount of semen can also be measured (this is called the motile density).
  • pH. This is a measure of the acidity (low pH) or alkalinity (high pH) of the semen.
  • White blood cell count. White blood cells are not normally present in semen.
  • Fructose level. This is a measure of the amount of a sugar, called fructose, in the semen. The fructose provides energy for the sperm.

This test is done on a sample of semen.

Semen Analysis
Results

Usually results of a semen analysis are available within a day. Normal values may vary from lab to lab.

Semen volume

Normal:

1.0–6.5 milliliters (mL) per ejaculation

Abnormal:

An abnormally low or high semen volume may sometimes cause fertility problems.

Liquefaction time

Normal:

less than 60 minutes

Abnormal:

An abnormally long liquefaction time may indicate an infection.

Sperm count

Normal:

20–150 million sperm per milliliter (mL)

0 sperm per milliliter if the man had a vasectomy

Abnormal:

If the sperm count is very low, a man may be infertile. However, a low sperm count does not always mean that a man cannot father a child. Men with sperm counts below 1 million have fathered children.

Sperm shape (morphology)

Normal:

At least 60% of the sperm have normal shape and structure.

Abnormal:

Sperm can be abnormal in several ways, such as having two heads or two tails, a short tail, a tiny head (pinhead), or a round (rather than oval) head. Abnormal sperm may be unable to move normally or to penetrate an egg. Some abnormal sperm are usually found in every normal semen sample. However, a high percentage of abnormal sperm may impair a man's ability to father a child.

Sperm movement (motility)

Normal:

At least 60% of the sperm show normal forward movement.

At least 8 million sperm per milliliter (mL) show normal forward movement.

Abnormal:

Sperm must be able to move forward ("swim") through cervical mucus to reach an egg. If a high percentage of sperm cannot swim properly, the man may find it difficult to father a child.

Semen pH

Normal:

Semen pH of 7.1–8.0

Abnormal:

An abnormally high or low semen pH can kill sperm or affect their ability to move or to penetrate an egg.

White blood cells

Normal:

No white blood cells or bacteria are detected.

Abnormal:

The presence of bacteria or a large number of white blood cells in semen can indicate an infection.

Fructose level

Normal:

300 milligrams (mg) fructose per 100 milliliters (mL) of ejaculate

Abnormal:

The absence of fructose in the semen may indicate that the man was born without seminal vesicles or has blockage of the seminal vesicles.

If a low sperm count or a high percentage of sperm abnormalities are found, further testing may be done. Other tests may include measuring hormones, such as testosterone, luteinizing hormone (LH), follicle-stimulating hormone (FSH), or prolactin. A small sample (biopsy) of the testicles may be needed for further evaluation if the sperm count or motility is extremely low.

 
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